C2397-26A

CD43 (T Cell Marker, Leukosialin, Sialophorin, or Leukocyte Sialoglycoprotein)

Size

£479.00

SKU:: C2397-26A
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Human KG-1 tumor cells
Clone:: 7H182 (DFT1)
Format:: Supplied as a liquid in PBS, pH 7.2, 0.5mg/ml gentamicin sulfate.
Extra Details:: Human CD43 is a heavily O-glycosylated and sialylated transmembrane protein that is mainly found on T cells, but not on B cells. Applications: Suitable for use in Flow Cytometry. Immunohistochemistry and Western Blot. Other applications not tested. Recommended Dilutions: Flow Cytometry: 10ug/ml (80ul) Immunohistochemistry: Fix cells or tissues Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. Five x10e5 cultured Jurkat cells/tube were incubated 45 minutes on ice with 80ul of C2397-26A at 10ug/ml. 2. Cells were washed twice and incubated with Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. 3. Cells stained positive with a mean shift of 1.71 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.