C2397-23C

CD43 (Galactoglycoprotein, GALGP, GPL115, Leukocyte sialoglycoprotein, Leukosialin, LSN, Sialophorin)

Size

£479.00

SKU:: C2397-23C
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Human KG-1 tumor cells
Clone:: DFT1
Format:: Supplied as a liquid in PBS. Preservative free
Extra Details:: Human CD43 is a heavily O-glycosylated and sialylated transmembrane protein that is mainly found on T cells, but not on B cells. Applications: Suitable for use in Western Blot, Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry: Fixed cells or tissues Flow Cytometry: 10ug/ml (80ul) Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. Five x10e5 cultures Jurkat cells/tube were incubated 45 minutes on ice with 80ul of C2397-23c at 10ug/ml. 2. Cells were washed twice and incubated with Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. 3. Cells stained positive with a mean shift of 1.71 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.