C2395-20B
CD42b alpha (Glycoprotein Ib alpha, GPIba)
Size
£806.00
- SKU:
- C2395-20B
- Purity:
- Purified by Protein G affinity chromatography from cell culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to His17-Leu505 from human CD42b alpha, expressed in NS0 cells.
- Clone:
- 8H211
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Extra Details:
- GPIb-alpha (platelet glycoprotein Ib-alpha; also CD42b and glycocalicin) is a platelet-derived 145kD member of the leucine-rich glycoprotein family of molecules. Mature human GPIb-alpha is a type I transmembrane protein 610 amino acids (aa) in length. It contains a 489 aa extracellular domain (ECD) (aa 17-505) and a 100 aa cytoplasmic region (aa 527-626). As part of a membrane complex, the GPIb-alpha ECD binds to von Willebrand Factor to impede platelet flow and promote clotting. The human GPIb-alpha ECD is approximately 50% aa identical to dog and mouse GPIb-alpha ECD. Applications: Suitable for use in Flow Cytometry, Direct ELISA and Western Blot. Other applications not tested. Recommended Dilutions: Flow Cytometry: 0.25ug/10e6 cells using human CD41+ platelets Western Blot: 2ug/ml using human platelets Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice