C2394-58

CD42a, gpIb, IX

Size

£563.00

SKU:: C2394-58
Purity:: Purified by Protein A affinity chromatography from tissue culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Peripheral blood mononuclear cells isolated on Ficoll hypaque.
Clone:: FMC-25
Format:: Supplied as a liquid in PBS, 0.09% sodium azide.
Extra Details:: CD42a cell surface antigen is a 22kD glycoprotein also known as the platelet glycoprotein gp Ib-IX complex. CD42a is expressed by platelets and megakaryocytes. Has been successfully used as a capture reagent for platelet-autoantibody complexes in the sera of patients presenting thrombocytopenia associated with antiphospholipid syndrome (Godeau et al. 1997). Applications: Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 1:50-1:200. Dilute in PBS. Use 50ul of diluted antibody per 1x10e6 platelets in 100ul of buffer. Immunohistochemistry: frozen sections Optimal working dilutions to be determined by researcher. Immunohistochemistry Recommended Positive Control Tissue: Bone marrow. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.