C2392-17F

CD40 (PE/CY5)

Size

£524.00

SKU:: C2392-17F
Purity:: Purified by affinity chromatography.
Storage Conditions:: 4°C Do not freeze
Supplier:: United States Biological
Host:: Rat
Clone:: 5K254 (1C10)
Format:: Supplied as a liquid in PBS, 0.09% sodium azide and a stabilizing agent. Labeled with PE/CY5.
Extra Details:: CD40 is a type I cell surface protein belonging to the tumor necrosis factor superfamily of cell surface receptors. In mice it is expressed on B lineage cells, follicular dendritic cells, thymic epithelium, and interdigitating cells in the T-cell zone of secondary lymphoid organs.(1-5) CD40 first becomes detectable on a subset of small pre-B II cells in bone marrow, with the levels of CD40 expression increasing thereafter during B-cell maturation. Immature B cells (IgM+IgDloB220lo) express intermediate levels of CD40, whereas mature B cells (IgM+IgDhiB220hi) express high levels.4 CD40 has a central role in B cell growth and differentiation, and signalling through CD40 in combination with IL-4 reportedly induces immunoglobulin isotype switching and secretion of IgE.6 The agonistic monoclonal antibody closely resembles gp39/CD40 ligand in its ability to stimulate proliferation of small, resting B lymphocytes in the absence of other cofactors. PE/CY5 combination conjugate is used as a third fluorochrome in multicolor flow cytometry applications when only a single 488nm excitation source is available. Antibodies are covalently coupled using heterobifunctional linking reagents, resulting in an optimal conjugation format for flow cytometry. Applications: Suitable for use in Flow cytometry, Immunoprecipitation, Immunofluorescence and In vitro induction of proliferation of mature B lymphocytes. Other applications have not been tested. Recommended Dilution: Flow Cytometry: ≤ 0.5ug/10e6 cells Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity.

Shipping Conditions:: Blue Ice