C2392-03K
CD40 (PE)
Size
£730.00
- SKU:
- C2392-03K
- Application:
- Flow Cytometry
- Purity:
- Purified by Protein G affinity chromatography from tissue culture supernatant.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Rat
- Immunogen:
- Extracellular domain of mouse CD40 and the Fc portion of human IgG1.
- Clone:
- 3/23
- Format:
- Supplied as a lyophilized powder from PBS, 1% BSA, 5% sucrose, 0.09% sodium azide. Labeled with R-Phycoerythrin (PE). Reconstitute with 1ml sterile ddH2O.
- Extra Details:
- The specificity of CD40 antibody was demonstrated by ELISA and flow cytometry using BHK cells transfected with mouse CD40. CD40 is a powerful activator of normal B cells especially in the presence of IL-4. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: Neat. 10ul labels 10e6 cells in 100ul Optimal dilutions to be determined by the researcher. Hybridoma: Ag8 myeloma cells with spleen cells from LOU/c rats. Storage and Stability: Store product at 4°C before and after reconstitution. DO NOT FREEZE! Lyophilized and reconstituted products are stable for 12 months at 4°C after receipt. Reconstitute lyophilized powder with sterile ddH2O prior to use and store remainder as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product centrifuge the original vial prior to removing the cap.
- Shipping Conditions:
- Blue Ice