C2392-03
CD40
Size
£691.00
- SKU:
- C2392-03
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- CD40 Ig(Fc) fusion protein containing the EC region of human CD40 and the Fc region of human IgG.
- Clone:
- 3H1283 (LOB7/6)
- Format:
- Supplied as a liquid in TBS, pH 7.4, 0.09% sodium azide.
- Extra Details:
- CD40 cell surface antigen is a 48kD glycoprotein expressed by B lymphocytes and weakly by some monocytes. CD40 is involved in the process of B cell selection in germinal centers and is vital in T cell-B cell interactions. Applications: Suitable for use in Flow Cytometry, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 20ug/ml. Use 10ul to label 10e6 cells in 100ul. Immunohistochemistry (Frozen and paraffin): 1-10ug/ml. Requires protein digestion pre-treatment of paraffin embedded sections prior to staining. Immunocytochemistry: 1:10ug/ml Optimal dilutions to be determined by the researcher. Immunohistochemsitry Positive Control: Human tosil Hybridoma: Spleen cells from immunized BALB/c mice were fused with cells of the mouse NS1 myeloma cell line. Recommended Secondary Reagents: I1904-65W: IgG, F(ab’)2 (HRP) Pab RbxMo I1904-66C: IgG, F(ab’)2, X-Adsorbed (HRP) Pab RbxMo I1904-65V IgG, F(ab’)2 (FITC) Pab RbxMo I1904-66A IgG, F(ab’)2, X-Adsorbed (FITC) Pab RbxMo F(ab')2 rabbit anti mouse IgG: PE conjugate Recommended Negative Controls: I1904-83P1: IgG2a Murine Negative Control Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice