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C2391-30

CD39L3 (CD39-like 3, Ectonucleoside Triphosphate Diphosphohydrolase-3, eNTPDase 3, HB6) (Biotin)

Size

£908.00

SKU:
C2391-30
Application:
WB
Purity:
Purified by immunoaffinity chromatography.
Storage Conditions:
-20°C
Supplier:
United States Biological
Host:
Sheep
Immunogen:
CHO cell-derived rmCD39L3, (aa44-485)
Format:
Supplied as a lyophilized powder from PBS, pH 7.4, BSA. Labeled with Biotin. Reconstitute with 250ul sterile PBS.
Extra Details:
CD39L3 (CD39-like 3, Ectonucleoside Triphosphate Diphosphohydrolase-3, eNTPDase 3, HB6) is an 80kD, class I member of the GDA1/CD39 NTPase family of transmembrane (TM) enzymes. It is a cell-surface glycoprotein that hydrolyses both extracellular ATP and ADP. Thus, it controls ATP activation of P2X ion channel purinoreceptors. Mouse CD39L3 is 529 amino acids (aa) in length. It contains two TM segments plus an N- and C-terminal cytoplasmic region. Based on human CD39L3, the mouse extracellular domain (ECD) will contain aa 44-485. Within the ECD, mouse CD39L3 shares 82% and 94% aa identity with human and rat CD39L3 ECD, respectively. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.1-0.2ug/ml detection limit for rmCD39L3 is ~5ng/lane under non-reducing and reducing conditions. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Shipping Conditions:
Blue Ice