C2391-01L
CD39 (ATPDase, CD39, DKFZp686D194, DKFZp686I093, Ecto-apyrase, Ecto-ATPase 1, Ecto-ATPDase 1, Ecto-ATP diphosphohydrolase 1, Ectonucleoside triphosphate diphosphohydrolase 1, FLJ40921, FLJ40959, Lymphoid cell activation antigen, NTPDase 1, NTPDase-1)
Size
£479.00
- SKU:
- C2391-01L
- Purity:
- Purified by Protein A affinity chromatography from tissue culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Human WM-1 (Waldenström's macroglobulinemia) cell line.
- Clone:
- BU61
- Format:
- Supplied as a liquid in 50mM sodium phosphate, pH 7.5, 100mM potassium chloride, 150mM sodium chloride. No preservative added.
- Extra Details:
- Human CD39 is found on most mature B cells, activated NK cells and activated T cells. CD39 has homology to the N- myc family of proteins and has been cloned. Applications: Suitable for use in Western Blot, Flow Cytometry, Immunohistochemistry and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Flow Cytometry: 5ug/ml (80ul) using 5x10e5 ficoll prepared huma peripheral blood mononuclear cells. Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. 5x10e5 ficoll prepared human peripheral blood mononuclear cells were washed and pre-incubated for about 5 minutes with 20ul of 300ug/ml human IgG (to block non specific binding) after which they were incubated 45 min on ice with 80ul of C2391-01L (5ug/ml). 2. Cells were washed twice and incubated with reagent IgG goat anti-mouse (FITC), after which they were washed three times, fixed and analyzed by FACS. 3. A 15% sub population of the cells stained positive with a mean shift of 1.25 log10 fluorescent units when compared to a mouse IgG1 negative control at a similar concentration. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice