C2391-01D
CD39 (Apyrase, ATPDase, EC3.6.1.5, Ecto-Apyrase,Ecto-ATPase, ENTPD1, Ectonucleoside triphosphate diphosphohydrolase 1, NTPDase-1, Nucleoside triphosphate diphosphohydrolase 1, Vascular ATP diphosphohydrolase) (PE)
Size
£607.00
- SKU:
- C2391-01D
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Human WM-1 (Waldenström's macroglobulinemia) cell line.
- Clone:
- 7H171
- Format:
- Supplied as a liquid in 50mM sodium phosphate, pH 7.5, 500mM potassium chloride, 150mM sodium chloride, 0.2% BSA, 0.04% sodium azide, 15% glycerol. Labeled with R-Phycoerythrin (PE).
- Extra Details:
- Human CD39 is found on most mature B cells, activated NK cells and activated T cells. CD39 has homology to the N- myc family of proteins and has been cloned. Applications: Suitable for use in Western Blot, Flow Cytometry, Immunohistochemistry and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Flow Cytometry: 5ug/ml (80ul) using 5x10e5 ficoll prepared huma peripheral blood mononuclear cells. Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. 5x10e5 ficoll prepared human peripheral blood mononuclear cells were washed and pre-incubated for about 5 minutes with 20ul of 300ug/ml human IgG (to block non specific binding) after which they were incubated 45 min on ice with 80ul of C2391-01L (5ug/ml). 2. Cells were washed twice and incubated with reagent IgG goat anti-mouse (FITC), after which they were washed three times, fixed and analyzed by FACS. 3. A 15% sub population of the cells stained positive with a mean shift of 1.25 log10 fluorescent units when compared to a mouse IgG1 negative control at a similar concentration. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
- Shipping Conditions:
- Blue Ice