C2390-03D
CD38 (ADP-ribosyl cyclase 1, cADPr hydrolase 1, Cyclic ADP-ribose hydrolase 1, T10)
Size
£479.00
- SKU:
- C2390-03D
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Human T cell tumor cells: CEM
- Clone:
- AT1
- Format:
- Supplied as a liquid in 50mM sodium phosphate, pH 7.5, 100mM potassium chloride, 150mM sodium chloride. No preservative added.
- Extra Details:
- Human CD38 is a type II transmembrane glycoprotein with bifunctional ectoenzyme activity catalyzing both synthesis and hydrolysis of cyclic ADP ribose. CD38 is involved in lymphocyte activation and adhesion to endothelium and has recently been identified as a counter-receptor of CD31. Applications: Suitable for use in Flow Cytometry and Western Blot. Other applications not tested. Recommended Dilution: Flow Cytometry: 5ug/ml labels 5x10e5 cultured Raji cells Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. 5x10e5 cultured Raji cells per tube were washed and incubated 45 minutes on ice with 80ul of C2390-03D at 5ug/ml. 2. Cells were washed twice and incubated with and IgG (FITC) goat anti-mouse, after which they were washed three times, fixed and analyzed by FACS. 3. Cells stained positive with a mean shift of 1.71 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice