C2390-01B-BIOTIN

CD38 (Acute Lymphoblastic Leukemia Cells Antigen, ADP Ribosyl Cyclase, CADPr Hydrolase 1, Cyclic ADP Ribose Hydrolase, Ecto Nicotinamide Adenine Dinucleotide Glycohydrolase, I19 (Mouse), Lymphocyte Differentiation Antigen) (Biotin)

Size

£940.00

SKU:: C2390-01B-BIOTIN
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: CD38
Clone:: 3H1272 (AT13/5)
Format:: Supplied as a liquid in PBS, pH 7.2. Labeled with Biotin.
Extra Details:: The CD38 antigen is also known as T10 antigen. The CD38 antigen is a 45kD single-chain type II glycoprotein., It is an integral membrane protein with a long extracellular C-terminal domain, a single membrane-spanning region and a short N-terminal cytoplasmic tail. The CD38 antigen is expressed on a variety of hematopoietic cells. Its distribution depends on the state of the cell differentiation and the cell activation. In adults, the CD38 molecule is expressed on earlier stage of B lymphocyte ontogeny, lost during maturation and re-expressed upon terminal differentiation to plasma cells. This molecule is also strongly expressed on thymocytes. It is found at low density on resting T lymphocytes. It is expressed on the majority of resting NK cells and monocytes. It is also found on platelets and red blood cells. Applications: Suitable for use in Flow Cytometry, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:100. Use 10ul to label 10e6 cells in 100ul. Immunohistochemistry: Frozen Optimal dilutions to be determined by the researcher. Hybridoma: NS1 myeloma cells with spleen cells from Balb/c mice. Recommended Negative Controls: Mouse IgG1 Negative Control Recommended Secondary Reagents: F(ab')2 rabbit anti-mouse IgG HRP F(ab')2 rabbit anti mouse IgG: RPE conjugate Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile (40-50% ) glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Applications are based on unconjugated antibody.

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