C2389-06C
CD37 (TSPAN26) (FITC)
Size
£557.00
- SKU:
- C2389-06C
- Application:
- Flow Cytometry
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Human spleen cells from a patient with hairy cell leukemia
- Clone:
- 7H197
- Format:
- Supplied as a liquid in PBS, pH 7.5, 0.2% BSA, 0.04% sodium azide, 5% glycerol. Labeled with fluorescein isothiocyanate (FITC).
- Extra Details:
- Human CD37 is a member of the tetraspan family of cell surface molecules that apparantly are involved in signal transduction or transporter system complexes. CD37 is strongly expressed on B lymphocytes (sIg-positive) and weakly on T cells, neutrophils and monocytes. Expression of CD37 disappears during B cell development at the plasma cell stage. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:50 (10ug/ml) 80ul labels 5x10e5 cultured human Raji cells. Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. Five x 10e5 cultured human Raji cells per tube were washed and incubated 45 minutes on ice with 80ul of C2389-06C at a 1:50 dilution (10ug/ml). 2. Cells were washed three times, fixed and analyzed by FACS. 3. Cells stained positive with a mean shift of 1.73 log10 fluorescent units when compared to a Mouse IgG2b/FITC negative control at a similar concentration. 4. Binding was blocked when pre incubated 10 minutes with 20ul of 0.5mg/ml anti-CD37 antibody (C2389-06A). Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.
- Shipping Conditions:
- Blue Ice