C2389-01C

CD37 (TSPAN26)

Size

£825.00

SKU:: C2389-01C
Application:: Flow Cytometry
Purity:: Purified by Protein A affinity chromatography from hybridoma culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to human CD37, expressed in NS0 cells.
Clone:: 8H186
Format:: Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Extra Details:: CD37, also known as TSPAN26, is a 40-45kD palmitoylated tetraspanin superfamily glycoprotein that is expressed by lymphocytes and myeloid cells. CD37 directly associates with dectin-1 on macrophages and stabilizes cell surface dectin-1 expression. It also inhibits T cell activation and proliferation by limiting T cell receptor signaling. Human CD37 shares 80% aa sequence identity with mouse and rat CD37. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 25ug/ml; 10ul labels 1-2.5x10e5 cells in a total reaction volume not exceeding 200ul. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.