C2388-04B
CD36 (gpIIIb, gpIV, Thrombospondin Receptor) (Biotin)
Size
£557.00
- SKU:
- C2388-04B
- Purity:
- Purified by size exclusion chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Human tonsil cells /PBM.
- Clone:
- 7H216
- Format:
- Supplied as a liquid in 50mM sodium phosphate pH 7.5, 500mM potassium chloride, 150mM sodium chloride, 0.2% BSA, 0.04 sodium azide. Labeled with Biotin.
- Extra Details:
- Human CD36 is a major platelet glycoprotein also found on monocytes, macrophages and some endothelial cells. CD36 may play a role in cell adhesion as a receptor for thrombospondin. CD36 is a receptor for adhesion of malaria infected erythrocytes. CD36 is also proported to have a role in phagocytosis. Applications: Suitable for use in Flow Cytometry and Immunoprecipitation. Other applications not tested. Recommended Dilution: Flow Cytometry: 10ug/ml; 80ul labels 5x10e5 cells. Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. 5x10e5 ficoll prepared human peripheral blood mononuclear cells (PBMC) were washed and pre incubated ~5 minutes with 20ul of 250ug/ml human IgG (to block non specific binding) 2. They were then incubated 45 minutes on ice with 80ul of C2388-04B at 10ug/ml. 3. Cells were washed twice and incubated with secondary reagent, S7973-88P Streptavidin (PE). 4. They were then washed three times, fixed and analyzed by FACS analysis. 5. A 7.5% sub population of cells stained positive with a mean shift of 2.3log10 fluorescent units when compared to a Mouse IgM/Biotin negative control at a similar concentration. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice