C2387-04C
CD35 (CR1, Follicular Dendritic Cell Marker) (Biotin)
Size
£557.00
- SKU:
- C2387-04C
- Purity:
- Purified by Protein A affinity chromatography from tissue culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Human monocytes.
- Clone:
- 7H183
- Format:
- Supplied as a liquid in 50mM sodium phosphate, pH 7.5, 100mM potassium chloride, 150mM sodium chloride, 0.2% BSA, 0.04% sodium azide, 5% glycerol.
- Extra Details:
- Human CD35 binds complement components C3b , C4b, and iC3b and is expressed on erythrocytes, B cells, monocytes, granulocytes and folicular dendritic cells and on subsets of thymocytes (3). CD35 on erythrocytes mediates clearance of immunue complexes. Applications: Suitable for use in Flow Cytometry and Western Blot. Other applications not tested. Recommended Dilutions: Flow Cytometry: 10ug/ml labels 5x10e5 cultured HPB-MLT cells Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. Five x 10e5 cultured HPB-MLT cells per tube were washed and incubated for 45 minutes on ice with 80ul of C2387-04C at 10ug/ml. 2. Cells were washed two times and incubated or 45 minutes with secondary reagent, fixed and analyzed by FACS. 3. Cells stained positive with a mean shift of 1.28 log10 fluorescent units when compared to a Mouse IgG1/Biotin negative control at a similar concentration. 4. Binding was blocked when cells were preincubated for 10 minutes with 20ul of 0.5mg/ml C2387-04B. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice