CD34 (Endothelial Cell Marker, Cluster Designation 34, Hematopoietic Progenitor Cell Antigen CD34, HPCA1)

Size

£895.00

SKU:: C2386-12A
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: Recombinant protein corresponding to Glu37-Ile293 from rat CD34 expressed in NS0 cells (NP_001100672). Species Sequence Homology: mouse, 83%; human, 57%
Format:: Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.
Extra Details:: CD34 is a 110-120kD type I transmembrane glycoprotein that belongs to the CD34/Podocalyxin family of sialomucins. It is a widely used marker of activated hematopoietic stem/progenitor cells and is also expressed on adult vascular endothelial cells. Mature rat CD34 is predicted to be a 352aa protein with a 257 aa extracellular domain (ECD) (aa35-291) and 74aa cytoplasmic tail (aa313-386). Over the region used as immunogen, rat CD34 shares 83% and 57% aa identity with mouse and human CD34, respectively. Applications: Suitable for use in Immunohistochemistry, Western Blot and Direct ELISA. Other applications not tested. Recommended Dilutions: Immunohistochemistry: 5-15ug/ml stains perfusion fixed frozen sections of rat liver. Western Blot: 0.1ug/ml stains recombinant rat CD34. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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