C2386-02Q

CD34 (Endothelial Cell Marker, Cluster Designation 34, Hematopoietic Progenitor Cell Antigen CD34, HPCA1) (PE)

Size

£730.00

SKU:: C2386-02Q
Application:: Flow Cytometry
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Rat
Immunogen:: Mouse transformed endothelioma cell line tEnd.
Clone:: MEC14.7
Format:: Supplied as a lyophilized powder from PBS, pH7.4, 0.09% sodium azide and 1% BSA. Labeled with R. Phycoerythrin (PE). Reconstitute with 1ml ddH2O.
Extra Details:: CD34 antigen is expressed on haematopoietic progenitor cells (1-3), vascular endothelium, and some tissue fibroblasts (4,5). CD34 antibodies are important in experimental and clinical studies on human haemopoietic progenitors, candidate stem cells, and leukemic cell populations. CD34 antibodies are used clinically for bone marrow manipulation. It appears that the glycosylation of CD34 differs between HEV, vascular endothelium or haemopoietic cell lines, suggesting the existence of HEV-specific glycoforms of CD34 that could function as HEV ligands of lymphocyte L-selectin in humans. This suggests that, in addition to its role in haemopoiesis, CD34 could also play a role in lymphocyte recirculation. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: Neat 1:5. Use 10ul to label 10e6 cells in 100ul. Optimal dilutions to be determined by the researcher. Recommended Negative Control: I1904-82H1: IgG2a, Rat Negative Control (PE) Storage and Stability: Store product at 4°C before and after reconstitution. DO NOT FREEZE! Lyophilized and reconstituted products are stable for 12 months at 4°C after receipt. Reconstitute lyophilized powder with sterile ddH2O prior to use and store remainder as an undiluted liquid. Caution: PE conjugates are sensitive to light. For maximum recovery of product centrifuge the original vial prior to removing the cap.

Shipping Conditions:: Blue Ice