C2386-02C

CD34 (Endothelial Cell Marker, Cluster Designation 34, Hematopoietic Progenitor Cell Antigen CD34, HPCA1)

Size

£745.00

SKU:: C2386-02C
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rat
Immunogen:: Murine transformed endothelioma cell line tEnd.
Clone:: MEC14.7
Format:: Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.
Extra Details:: CD34 is a single-pass type I membrane glycophosphoprotein present on small vessel endothelial cells and hematopoietic progenitor cells. The apparent molecular mass of CD34 is heterogeneous, depending on the glycosylation state in different cell types. In cultured endothelioma cell lysate, CD34 has a MW of ~100kD, whereas in lung lysates it is ~80kD. Two Isoforms of CD34 exist and both are expressed on the cell surface. CD34 is an adhesion molecule performing a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. CD34 acts as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. CD34 presents carbohydrate ligands to selectins. CD34 is widely used as a marker to select early hematopoietic stem and progenitor cells in experimental and clinical hematopoiesis. Applications: Suitable for use in ELISA, Immunofluorescence, Immunohistochemistry, Western Blot, Immunoprecipitation, Flow Cytometry and Cell Separation. Other applications have not been tested. Recommended Dilution: Immunohistochemistry (Frozen): 1:50 Immunohistochemistry (Paraffin): 5ug/ml; Boil twice for 5 minutes in citrate, pH 6.0, or 0.03% trypsin treatment for 10 minutes at 37°C. Incubation at 4°C overnight resulted in the specific staining of endothelial cells. Western Blot: 1:50; Both reduced and non-reduced, 70-105kD, depending on glycosylation state and cell type. Immunoprecipitation: 1:50; 100kD protein observed in H5V cells. Flow Cytometry: 0.1ug/10e6 cells (mice blood cells). Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice