C2385-01L4-ML550
CD33 (gp67, Myeloid Cell Surface Antigen CD33 Precursor, Sialic Acid Binding Immunoglobulin Like Lectin 3, Siglec 3) (MaxLight 550)
Size
£819.00
- SKU:
- C2385-01L4-ML550
- Purity:
- Purified by Protein G affinity chromatography from tissue culture supernatant.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Human AML cells
- Clone:
- 3H1234 (WM53)
- Format:
- Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™550.
- Extra Details:
- MaxLight™550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (550nm); Emission (575nm); Extinction Coefficient 150,000. CD33 is a 67kD molecule expressed by monocytes, granulocyte precursors, myeloid progenitor cells and myeloid leukemias. The CD33 antigen, considered to be specific for the myeloid linage, has been reported to be present on cells of lymphoid origin. CD33 antigen is a useful marker for the diagnosis of non lymphoblastic leukemias and also for distinguishing myelogenous leukemia cells from lymphoid leukemia cells. The CD33 antigen is earlier expressed in the cytoplasm (cyCD33) than the expression on the cell membrane (mCD33) of the myeloblasts. It is more valuable using cyCD33 and cyCD13 to diagnose leukemia of AML-M0 (as the CD13 antigen is also earlier expressed in the cytoplasm (cyCD13) than the expression on the cell membrane (mCD13) of the myeloblasts). The CD33 antigen functions as an adhesion molecule. Applications: Suitable for use in Flow Cytometry, Immunohistochemistry, Immunoprecipitation and Western Blot. Other applications have not been tested. Recommended Dilution: Immunohistochemistry (Frozen): Overnight incubation recommended. Not recommended for use with paraffin sections. Immunoprecipitation: precipitates a protein of ~75kD form myeloid cells and a ~67kD protein from lymphoid cells Optimal dilutions to be determined by the researcher. Immunohistochemistry Positive Control: Lymphatic tissue Recommended Negative Control: I1904-78R:IgG1 Murine Negative Control Hybridoma: NS1 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice