C2384-02B
CD32 (Fc gamma Receptor II, FcRII, Monocyte Activation Antigen gp40) (Biotin)
Size
£557.00
- SKU:
- C2384-02B
- Application:
- Flow Cytometry
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Human K562 tumor cells and L cells transfected with human Fc gamma RII
- Clone:
- 7H135
- Format:
- Supplied as a liquid in PBS, pH 7.5, 0.2% BSA, 0.04% sodium azide, 5% glycerol. Labeled with Biotin.
- Extra Details:
- Human CD32 exists in at least six isoforms originating from three different genes (FcgRII A, B and C). The CD32 molecule is a low affinity receptor for immune complexed IgG and has signal-transducing capabilities involved with humoral and cell-mediated immune responses. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 10ug/ml; 80ul labels 5x10e5 cells. Optimal dilutions to be determined by the researcher. Flow Cytometry Procotol: 1. 5x10e5 cultured human Raji cells were washed and incubated 45 minutes on ice with 80ul of C2384-02B at 10ug/ml. 2. Cells were washed twice and incubated with 2º reagent Streptavidin/R-PE (S7973-88P). 3. The cells were then washed three times, fixed and analyzed by FACS. 4. Cells stained positive with a mean shift of 2.26 log10 fluorescent units when compared to a Mouse IgG1/Biotin negative control at a similar concentration. 5. Binding was blocked when cells were pre-incubated 10 minutes with 20ul of 0.5mg/ml C2384-02A. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice