C2384-01C

CD32 (Fcg Receptor II) (FITC)

Size

£620.00

SKU:: C2384-01C
Application:: Flow Cytometry
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: K562 cell line
Clone:: 3H1232 (AT10)
Format:: Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.09% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
Extra Details:: Human CD32 antigen is a 40kD glycoprotein that acts as a low affinity receptor for IgG (also known as Fc gamma RII). The antigen mediates several functions including endocytosis, activation of secretion, cytotoxicity and immunomodulation. CD32 is expressed by B cells, monocytes, granulocytes and platelets. Applications: Suitable for use in Flow Cytometry. Other applications have not been tested. Recommended Dilutions: Flow Cytometry: Neat-1:10. Use 10ul to label 10e6 cells or 100ul whole blood. Optimal working dilutions to be determined by researcher. Hybridoma: NS1 myeloma cells with spleen cells from BALB/c mice. Recommended Negative Control: I1904-78R3: IgG1 Murine Negative Control (FITC) Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.