C2384-01-FITC
CD32 (Fcg Receptor II) (FITC)
Size
£940.00
- SKU:
- C2384-01-FITC
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- K562 cell line
- Clone:
- 3H1232 (AT10)
- Format:
- Supplied as a liquid in PBS, pH 7.2. Labeled with Fluorescein isothiocyanate (FITC).
- Extra Details:
- CD32 is a 40kD glycoprotein that acts as a low affinity receptor for IgG (also known as Fc gamma RII). The antigen mediates several functions including endocytosis, activation of secretion, cytotoxicity and immunomodulation. CD32 is expressed by B cells, monocytes, granulocytes and platelets. Applications: Suitable for use in Flow Cytometry, Immunohistochemistry, Immunoprecipitation and Functional assays. Other applications not tested. Recommended Dilutions: Flow Cytometry: 20ug/ml. Use 10ul to label 10e6 cells or 100ul whole blood. Immunohistochemistry (Frozen): 1:500-1:1000 Immunoprecipitation: 20ug/ml Functional assays: Sodium azide needs to be removed prior to assay. Optimal dilutions to be determined by the researcher. Positive Control for Immunohistochemistry: Lymph node Hybridoma: Spleen cells from immunized BALB/c mice were fused with cells of the mouse NS1 myeloma cell line. Recommended Negative Control: I1904-79G: IgG1, Mouse, Negative Control Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice