C2384-01-BIOTIN
CD32 (Fcg Receptor II) (Biotin)
Size
£940.00
- SKU:
- C2384-01-BIOTIN
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- K562 cell line
- Clone:
- 3H1232 (AT10)
- Format:
- Supplied as a liquid in PBS, pH 7.2. Labeled with Biotin.
- Extra Details:
- CD32 is a 40kD glycoprotein that acts as a low affinity receptor for IgG (also known as Fc gamma RII). The antigen mediates several functions including endocytosis, activation of secretion, cytotoxicity and immunomodulation. CD32 is expressed by B cells, monocytes, granulocytes and platelets. Applications: Suitable for use in Flow Cytometry, Immunohistochemistry, Immunoprecipitation and Functional assays. Other applications not tested. Recommended Dilutions: Flow Cytometry: 20ug/ml. Use 10ul to label 10e6 cells or 100ul whole blood. Immunohistochemistry (Frozen): 1:500-1:1000 Immunoprecipitation: 20ug/ml Functional assays: Sodium azide needs to be removed prior to assay. Optimal dilutions to be determined by the researcher. Positive Control for Immunohistochemistry: Lymph node Hybridoma: Spleen cells from immunized BALB/c mice were fused with cells of the mouse NS1 myeloma cell line. Recommended Negative Control: I1904-79G: IgG1, Mouse, Negative Control Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice