C2383-10N
CD31 (PECAM-1, Platelet gpIIa Molecule) (FITC)
Size
£620.00
- SKU:
- C2383-10N
- Application:
- Flow Cytometry
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Clone:
- WM59
- Format:
- Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. Labeled with Fluorescein isothiocyanate (FITC).
- Extra Details:
- CD31 (PECAM-1, Platelet gpIIa Molecule), a ~130kD integral membrane glycoprotein, is expressed in platelets, cultured endothelial cells, monocytes, normal tissue macrophages in tonsil, lung and kidney, skin biopsies from conditions such as sarcoidosis and lepromatous leprosy and bone marrow-derived hematopoietic stem cells and embryonic stem cells. CD31 mediates cell-cell adhesion. Valuable iin the study of benign and malignanat vaascular tumors. Stainiing for CD31 has also been used to measure angiogenesis, which reportedly predicts tumor recurrence. FITC: Protein (molar ratio): 4.4: 1 Recommended Negative Controls: Mouse IgG1 Negative Control: FITC Applications: Flow Cytometry: Neat Optimal working dilutions to be determined by researcher. Flow Cytometry: Use 10ul of the suggested working dilution to label 10e6 cells or 100ul whole blood. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice