C2383-10A-PE
CD31 (PECAM-1, Platelet gpIIa Molecule) (PE)
Size
£852.00
- SKU:
- C2383-10A-PE
- Purity:
- Purified by Protein A affinity chromatography from tissue culture supernatant.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Activated, Lewis rat derived microglial cells.
- Clone:
- 3H1217 (TLD-3A12)
- Format:
- Supplied as a liquid in PBS, pH 7.2. Labeled with R-Phycoerythrin (PE).
- Extra Details:
- CD31 (PECAM-1, Platelet gpIIa Molecule), a ~130kD integral membrane glycoprotein, is expressed in platelets, cultured endothelial cells, monocytes, normal tissue macrophages in tonsil, lung and kidney, skin biopsies from conditions such as sarcoidosis and lepromatous leprosy and bone marrow-derived hematopoietic stem cells and embryonic stem cells. CD31 mediates cell-cell adhesion. Valuable in the study of benign and malignanat vaascular tumors. Stainiing for CD31 has also been used to measure angiogenesis, which reportedly predicts tumor recurrence. Applications: Suitable for use in Flow Cytometry, Immunohistochemistry, Immunofluorescence, FLISA and Western Blot. Other applications not tested. Recommended Dilutions: Flow Cytometry: 1:10-1:100. Use 10ul of diluted antibody to label 10e6 cells in 100ul. Immunohistochemistry (Frozen): 1:10-1:100 Immunohistochemistry (paraffin): Staining of endothelium may be enhanced on FFPE tissue by pre-treatment with 0.2M boric acid, pH 7.0 ( see Wilson et al.) Functional Studies: Recommend the use of C2383-03G. Optimal working dilutions to be determined by researcher. Hybridoma: Sp2/myeloma cells with spleen cells from Balb/c mice. Recommended Negative Control: I1904-78Y: IgG1 Mouse Negative Control (rat tissues only). Recommended Secondary Reagents: I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice