C2383-01D

CD31 (PECAM-1, Platelet gpIIa Molecule) (PE)

Size

£704.00

SKU:: C2383-01D
Application:: Flow Cytometry
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Stimulated human leukocytes
Clone:: 7H107
Format:: Supplied as a liquid in PBS, pH 7.5, 15% glycerol, 0.2% BSA, 0.04% sodium azide. Labeled with R-Phycoerythrin (PE).
Extra Details:: Human CD31 is an adhesion molecule expressed on platelets, endothelial cells, leukocytes and their bone marrow precursors. CD31 plays a role in homophilic adhesion and heterophilic transendothelial migration. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Protocol: 5 x 10e5 cultured Jurkat cells were washed and incubated 45 minutes on ice with 80ul of C2383-01D at a 1:50 dilution (20ug/ml). Cells were washed three times, fixed and analyzed using a BD FACS Calibur. Cells stained positive with a mean shift of 1.75 log10 fluorescent units when compared to a Mouse IgG1/R-PE negative control at a similar concentration. Binding was 99% blocked when pre incubated 10 minutes with 20ul of 0.5mg/ml C2383-01A. Storage and Stability: May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only pror to immediate use. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity.