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C2381-10F

CD29 (Integrin beta 1) (FITC)

Size

£620.00

SKU:
C2381-10F
Application:
Flow Cytometry
Purity:
Purified by Protein G affinity chromatography from tissue culture supernatant.
Storage Conditions:
-20°C
Supplier:
United States Biological
Host:
Mouse
Immunogen:
Ocular melanoma cell line V+B2.
Clone:
3H1196 (4B7R)
Format:
Supplied as a liquid in PBS, pH 7.2, 1% BSA, 0.09% sodium azide. Labeled with Fluorescein isothiocyanate (FITC).
Extra Details:
CD29 represents the 130kD beta1 integrin subunit that forms heterodimer complexes with many distinct a subunits including CD49a-f, which comprise the very late antigen family (VLA-1 to VLA-6) of integrins, and CD51. The CD29 molecule has a broad tissue distribution. It is expressed on all leukocytes, although only at low levels on granulocytes. Integrin heterodimers containing CD29 mediate cell-cell and cell-matrix interactions. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry (Neat): Use 10ul to label 10e6 cells in 100ul. Optimal dilutions to be determined by the researcher. Recommended Negative Controls: I1904-78R3: IgG1 Murine Negative Control (FITC) Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Shipping Conditions:
Blue Ice