C2381-10

CD29 (Integrin beta 1)

Size

£691.00

SKU:: C2381-10
Purity:: Purified by Protein G affinity chromatography from tissue culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Purified human b1 integrin preparation from HT1080 fibrosarcoma cell extract.
Clone:: 12G10
Format:: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Extra Details:: CD29 is also known as beta1 integrin or VLA-4 subunit alpha. CD29 is a ~130kD under reducing, ~115kD under non-reducing conditions single pass type I transmembrane glycoprotein. CD29 acts as the common beta subunit of the heterodimeric very late antigens 1-6, complexing with CD49a-f respectively where it forms part of the receptors for laminin, collagen and fibronectin. the VLA heterodimers mediate cell-cell and cell-matrix interactions. Applications: Suitable for use in ELISA, Western Blot, Immunofluorescence, Immunoprecipitation and Flow Cytometry. Other applications not tested. Recommended Dilutions: ELISA: 10ug/ml Immunohistochemistry: frozen Flow Cytometry: 1:25-1:50 Optimal working dilutions to be determined by researcher. Hybridoma: X63/Ag8.653 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.