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C2381-10-ML650

CD29 (Integrin beta 1) (MaxLight 650)

Size

£940.00

SKU:
C2381-10-ML650
Purity:
Purified by Protein G affinity chromatography from tissue culture supernatant.
Storage Conditions:
4°C Do Not Freeze
Supplier:
United States Biological
Host:
Mouse
Immunogen:
Purified human b1 integrin preparation from HT1080 fibrosarcoma cell extract.
Clone:
3H1190
Format:
Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™650.
Extra Details:
MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000. CD29 is also known as beta1 integrin or VLA-4 subunit alpha. CD29 is a ~130kD under reducing, ~115kD under non-reducing conditions single pass type I transmembrane glycoprotein. CD29 acts as the common beta subunit of the heterodimeric very late antigens 1-6, complexing with CD49a-f respectively where it forms part of the receptors for laminin, collagen and fibronectin. the VLA heterodimers mediate cell-cell and cell-matrix interactions. Applications: Suitable for use in FLISA, Western Blot, Immunofluorescence, Immunoprecipitation and Flow Cytometry. Other applications not tested. Recommended Dilutions: Immunohistochemistry: frozen Optimal working dilutions to be determined by researcher. Hybridoma: X63/Ag8.653 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
Shipping Conditions:
Blue Ice