C2381-05C

CD29 (Integrin beta 1)

Size

£832.00

SKU:: C2381-05C
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to Gln21-Asp728 (predicted) of human Integrin Beta-1 expressed in CHO cells (P05556).
Clone:: 7H248
Format:: Supplied as a lyophilized powder from a 0.2um filtered solution in PBS, 5% trehalose. Reconstitute with 1ml sterile, ddH2O or sterile 40-50% glycerol.
Extra Details:: The Integrin Beta1 subunit, also known as VLA-Beta chain and CD29, associates with at least ten different Integrin alpha subunits. Applications: Suitable for use in Western Blot, Flow Cytometry, Immunocytochemistry and ELISA. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml using recombinant human Integrin beta 1/CD29 under non-reducing conditions only Flow Cytometry: 0.2mg/ml 10ul labels 1-2.5x 10e5 cells in ≤200ul. Immunocytochemistry: 10ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.