C2381-05-FITC

Integrin, beta1 (CD29, Very Late Antigen, VLAb1, Platelet gplla) (FITC)

Size

£1,117.00

SKU:: C2381-05-FITC
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Purified human beta 1 integrin preparation from HT1080 fibrosarcoma cell extract
Clone:: 2Q837 (12G10)
Format:: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).
Extra Details:: CD29 (integrin Beta-1/gpIIa) is a cell adhesion molecule appearing on platelets, as the common Beta subunit of the very late activation antigen (VLA), and as a component of various protein complexes binding to extracellular matrix proteins. The CD29 is expressed at the cell surface exclusively as part of a heterodimer, in association with one of at least nine different integrin alpha subunits (alpha1, alpha2, alpha3, alpha4, alpha5, alpha6, alpha7, alpha8, and alphav). With the exception of red blood cells and possible weak expression on granulocytes, CD29 is expressed on nearly all cell and tissue types. Applications: Suitable for use in Flow Cytometry, FLISA and Western Blot. Other applications have not been tested. Recommended Dilutions: Western Blot: Reacts with human beta 1 integrin subunit under non-reducing conditions only Optimal dilutions to be determined by the researcher. Positive Controls: Tonsil and human skin tissue Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.

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