C2380-16-ML490
CD28 (MaxLight 490)
Size
£960.00
- SKU:
- C2380-16-ML490
- Application:
- Flow Cytometry
- Purity:
- Purified by ion exchange chromatography following ammonium sulfate precipitation.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Human T lymphocytes
- Clone:
- 2Q1294 (15E8)
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™490.
- Extra Details:
- MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000. The ligand for CD28 is CD80 (B7) which is found on activated B cells suggesting a role for CD28 in T and B cell interaction. Applications: Suitable for use in Flow Cytometry, T cell proliferation and in cell stimulation studies where it has been suggested that measuring T cell function in HIV infected asymptomatic patients may be an early indicator of AIDS, independent of CD4+ cell counts. Other applications have not been tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Hybridoma: Sp2/0 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice