C2380-09L
CD28 (PE)
Size
£730.00
- SKU:
- C2380-09L
- Application:
- Flow Cytometry
- Purity:
- Purified by Ion Exchange chromatography.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Rat
- Immunogen:
- Human peripheral blood T-cells.
- Clone:
- 6A266 (YTH913.12)
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.2, 0.09% sodium azide, 1% BSA. Labeled with R. Phycoerythrin (RPE). Reconstitute with 1ml sterile 40-50% glycerol, ddH2O.
- Extra Details:
- CD28 is a T-cell surface homodimeric 44kD molecule that belongs to the Ig superfamily(1). CD28 is involved in cell adhesion T-B through the binding to its ligand B7/BB1, which is also the ligand for CTLA- 4. CD28-B7 interaction is an important cosignal that allow T-cell proliferation and lymphokine production. CD28 is associated to a PI3 kinase activity (2). The majority of CD4+ T-cells and 50% of CD8+ T-cells express CD28 (3). Monoclonal antibody induces T-cell proliferation in costimulation with CD2 monoclonal antibodies (2). It also inhibits the CD4+ proliferation in the allogeneic T-cell response (2). This antibody has been studied at the Vth International Workshop on Human Leukocyte Differentiation Antigens in Boston (1993). Fusion Partners: Spleen cellsfrom an immunized DA rat were fused with cells of the Y3/Ag 1.2.3 rat myeloma cell line. Recommended Negative Controls: Rat IgG2b Negative Control: RPE Applications: Flow Cytometry: Neat Optimal working dilutions to be determined by researcher. Flow Cytometry: Use 10ul of the suggested working dilution to label 10e6 cells or 100ul human whole blood.
- Shipping Conditions:
- Blue Ice