C2380-03F
CD28 IgG2a Fc, Fusion Protein (Biotin)
Size
£832.00
- SKU:
- C2380-03F
- Molecular Weight:
- 41.8
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Format:
- Supplied as a liquid in PBS, pH 7.5, 0.2% BSA, 5% glycerol, 0.04% sodium azide. Labeled with Biotin.
- Extra Details:
- Human CD28 is an important costimulatory molecule found on all CD4+ T cells and on about half of the CD8+ T cells. T cell activities attributed to CD28 include, prevention of anergy, induction of cytokine gene transcription, stabilization of cytokine mRNAs and activation of CD8+ cytotoxic T lymphocytes. The ligands for CD28, identified as CD80 (B7-1) and CD86 (B7-2), are immunoglobulin superfamily monomeric transmembrane glycoproteins of 60kD and 80kD, respectively. Molecular Structure: A soluble fusion protein consisting of the extracellular (134 aa) domain of human CD28 fused to murine IgG2a Fc (232 aa), with a predicted monomeric molecular weight of 41.8kD. Transfectant Cell Line: CHO Human CD28-muIg/Biotin Fusion Protein Functional Application: CD28-muIg fusion protein blocks binding of anti-human CD28 to human tumor cells. Applications: Suitable for use in ELISA and Antibody Blocking. Other applications not tested. Recommended Dilution ELISA: 0.5ng/ml detects the fusion protein using an anti-CD28 antibody coated capture plate and Streptavidin/HRP as a detector. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Stable for 12 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice