C2380-03AX
CD28 (Azide Free)
Size
£1,272.00
- SKU:
- C2380-03AX
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Human CD28 Ig fusion protein and HPB-MLT human tumor cells
- Clone:
- 7H132
- Format:
- Supplied as a liquid in PBS, pH 7.2, 5% glycerol, 0.2% BSA.
- Extra Details:
- Human CD28 is an important costimulatory molecule found on all CD4+ T cells and on about half of the CD8+ T cells. T cell activities attributed to CD28 include prevention of anergy, induction of cytokine gene transcription, stabilization of cytokine mRNA and activation of CD8+ cytotoxic T lymphocytes. The ligands for CD28 identified as CD80 (B7-1) and CD86 (B7-2), are immunglobulin superfamily monomeric transmembrane glycoproteins of 60kD and 80kD respectively. Applications: Suitable for use in ELISA and Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 5ug/ml. 80ul labels 5x10e5 cultured HPB-MLT cells Optimal dilutions to be determined by the researcher. Flow Cytometry Procotol: 1. 5x10e5 cultured HPB-MLT cells were wash and incubated for 45 minutes on ice with 80ul of C2380-03AX at 5ug/ml. 2. Cells were washed twice and incubated with 50ul of IgG (FITC) goat anti-mouse. 3. Wash three times, fixed and analyzed by FACS. 4. Cells stained positive with a mean shift of 1.73 log10 fluorescent units when compared to a Mouse IgG1/R-PE negative control at a similar concentration. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice