C2380-01L

CD28 (MGC138290, T-Cell-Specific Surface Glycoprotein CD28, Tp44, TP44)

Size

£576.00

SKU:: C2380-01L
Purity:: Purified by Protein A affinity chromatography from tissue culture supernatent.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Human CD28 Ig fusion protein and HPB-MLT human tumor cells
Clone:: ANC28.1/5D10
Format:: Supplied as a liquid in 50mM sodium phosphate, pH 7.5, 100mM potassium chloride, 50mM sodium chloride. No preservative added.
Extra Details:: Human CD28 is an important costimulatory molecule found on all CD4+ T cells and on about half of the CD8+ T cells. T cell activities attributed to CD28 include prevention of anergy, induction of cytokine gene transcription, stabilization of cytokine mRNA and activation of CD8+ cytotoxic T lymphocytes. The ligands for CD28 identified as CD80(B7-1) and CD86(B7-2), are immunoglobulin superfamily monomeric transmembrane glycoproteins of 60kD and 80kD respectively. CD28 antibodies have recently been shown to have a strong adjuvant effect when conjugated to the immunogen (4). Applications: Suitable for use in ELISA and Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 5ug/ml Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. Five x 10e5 cultured HPB-MLT human tumor cells were washed and preincubated 5 minutes with 20ul of 0.5mg/ml human IgG (to block nonspecific binding). 2. After which they were incubated 45 minutes on ice with 80ul of C2380-01L at 5ug/ml. 3. Cells were washed twice and incubated with S7973-88P: Streptavidin (PE), after which they were washed three times, fixed and analyzed using FACS. 4. A net 65% subpopulation of the cells stained positive with a mean shift of 1.18 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration. Binding was blocked when cells were pre incubated 10 minutes with 20ul of 0.5mg/ml of C2418-02C. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice