CDT1 (CDT, CDT-1, Chromatin Licensing and DNA Replication Factor 1, DUP, hDUP, human homolog of Drosophila Double Parked, RIS2) (Not for Export EU)

Size

£800.00

SKU:: C2300-01A
Purity:: Serum
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to aa238-546 of human CDT1. Cellular Localization: Nucleus. Epitope: C-terminal half.
Format:: Supplied as liquid, 0.05% sodium azide.
Extra Details:: CDT1 (Chromatin Licensing and DNA replication Factor 1) is an essential component of the cell cycle machinery. It plays an important role in insuring that there is only one round of DNA replication per cell cycle. CDT1 is a known substrate of Cdk2 and Cdk4 (Liu, E. 2004). It is presented in G1 of the cell cycle and is degraded as cells enter S phase (Liu, E. 2004). In higher eukaryotes, CDT1 is known to interact with Geminin, a protein that is degraded during mitosis, that inhibits it. CDT1 is marked for degradation by two E3 ligases: SCF complex and the CUL4-DDB1 complex (Sansam, 2006). Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 was used to detect CDT1 in Jurkat cell lysate. Optimal dilutions to be determined by the researcher. Positive Control: Jurkat Cell Lysate Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.