C2275-89A
CD25 (Tac antigen, p55, Interleukin 2 Receptor alpha, IL2Ra, T Cell Growth Factor Receptor)
Size
£479.00
- SKU:
- C2275-89A
- Purity:
- Purified
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rat
- Immunogen:
- IL-2-dependent BALB/c mouse helper T-cell clone HT-2
- Clone:
- 7D4
- Format:
- Supplied as a liquid in 100mM borate buffered saline, pH 8.0. No preservatives or amine-containing buffer salts added.
- Extra Details:
- The IL-2 receptor (IL-2R) exists in three alternative forms made up from the individual components of CD25, CD122, and CD132. CD25 represents the low affinity alpha chain of the IL-2R, a type I transmembrane glycoprotein containing two CCP domains. It is rich in O-linked carbohydrates and has a short cytoplasmic tail.2 CD25 is expressed on activated T cells, B cells, NK cells and monocytes of all mouse strains tested.1, 3, 4 It is transiently expressed at a low level during B-cell development in bone marrow on the CD45R/B220low TdT- sIg- pre-B II and CD45R/B220low TdT- sIgM+sIgD- immature B cells, but not on the CD45R/B220low TdT+ sIg- pro-B/pre-B I stage nor on CD45R/B220high TdT- sIgM+sIgD+ mature B cells.5, 6 CD25 is expressed at a higher level during very early T-cell development in fetal and adult thymus.6, 7 While the antibody does not block the binding of IL-2 to CD25, when used in combination with C2275-82 it results in higher levels of inhibition of IL-2-driven proliferation than either of these two monoclonal antibodies alone.3, 8, 9 Applications: • Flow cytometry 1, 3, 4, 7, 8 • Immunoprecipitation 1, 3 Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice