C2275-86B-FITC

CD25 (Tac antigen, p55, Interleukin 2 Receptor alpha, IL2Ra, T Cell Growth Factor Receptor) (FITC)

Size

£940.00

SKU:: C2275-86B-FITC
Application:: Flow Cytometry
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Human PHA blasts; day 3 of culture.
Clone:: 3H1147 (MEM-181)
Format:: Supplied as a liquid in PBS, pH 7.2. Labeled with Fluorescein isothiocyanate (FITC).
Extra Details:: IL-2 receptor is expressed by activated lymphocytes. Applications: Suitable for use in Flow Cytometry. Other applications have not been tested. Recommended Dilution: Optimal working dilutions to be determined by researcher. Recommended Negative Controls: I1904-78R: Mouse IgG1 Negative Control Hybridoma: P3X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.