C2275-83C
CD25 (Tac antigen, p55, Interleukin 2 Receptor alpha, IL2Ra, T Cell Growth Factor Receptor) (FITC)
Size
£557.00
- SKU:
- C2275-83C
- Purity:
- Purified by Protein A affinity chromatography from tissue culture supernatant
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Stimulated human PBMC
- Clone:
- 7G7B6 (5)
- Format:
- Supplied as a liquid in 50mM sodium phosphate, 100mM potassium chloride, 150mM sodium chloride, pH 7.5, 0.2% BSA, 0.04% sodium azide, 5% glycerol. Labled with Fluorescein isothiocyanate (FITC)
- Extra Details:
- Expression of human CD25 (the alpha subunit of the IL-2 receptor complex) is dramatically upregulated on activated T cells, B cells, NK cells and macrophages. Antibodies to CD25 are being studied for potential use as an immundirected therapy to selectively attack (kill) CD25 expressing cells. Applications: Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 5ug/ml (1:50) labels 5x10e5 cells Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. Cultured human CEM were stimulated overnight in the presence of PMA (10ng/ml) and ionomycin (1mM). Five x 10e5 cells per tube were washed and incubated 45 minutes on ice with 80ul of C2275-83C (1:50). 2. Cells were washed three times, fixed and analyzed by FACS. 3. Cells stained positive with a mean shift of 1.12 log10 fluorescent units when compared to a mouse IgG2a (FITC) negative control at a similar concentration. 4. Binding was blocked when cells were pre incubated 10 minutes with 20ul of 0.5mg/ml of C2275-83A. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice