C2275-83A
CD25 (Tac antigen, p55, Interleukin 2 Receptor alpha, IL2Ra, T Cell Growth Factor Receptor)
Size
£479.00
- SKU:
- C2275-83A
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Stimulated PBMC
- Clone:
- 7H136
- Format:
- Supplied as a liquid in PBS, pH 7.2, 0.5mg/ml gentamicin sulfate.
- Extra Details:
- Expression of human CD25 (the alpha subunit of the IL-2 receptor complex) is dramatically upregulated on activated T cells, B cells, NK cells and macrophages (3). Antibodies to CD25 are being studied for potential use as an immundirected therapy to selectively attack (kill) CD25 expressing cells (4). Applications: Suitable for use in Flow Cytometry, Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry: Frozen sections Flow Cytometry: 5ug/ml Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. Cultured human CEM were stimulated overnight in the presence of PMA (10ng/ml) and ionomycin (1uM). 2. Five x 10e5 cells per tube were washed and incubated 45 minutes on ice with 80ul of aC2275-83A at 5ug/ml. 3. Cells were washed twice and incubated with secondary reagent Goat anti-Mouse IgG/FITC after which they were washed three times, fixed and analyzed by FACS. 4. Cells stained positive with a mean shift of 1.36 log10 fluorescent units when compared to a Mouse IgG2a negative control at a similar concentration. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice