C2275-82D

CD25 (Tac antigen, p55, Interleukin 2 Receptor alpha, IL2Ra, T Cell Growth Factor Receptor) (APC)

Size

£453.00

SKU:: C2275-82D
Purity:: Purified
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Rat
Clone:: 3C7
Format:: Supplied as a liquid in PBS, 0.09% sodium azide and a stabilizing agent. Labeled with Allophycocyanin (APC).
Extra Details:: The IL-2 receptor (IL-2R) exists in three alternative forms made up from the individual components of CD25, CD122, and CD132. CD25 represents the low affinity alpha chain of the IL-2R, a type I transmembrane glycoprotein containing two CCP domains. It is rich in O-linked carbohydrates and has a short cytoplasmic tail.14 CD25 is expressed on activated T cells, B cells, NK cells and monocytes of all mouse strains tested.2, 3, 15 Expression of CD25 on activated T lymphocytes is transitory and endogenously regulated.4 CD25 is expressed on precursor B cells in bone marrow. Its expression is initiated by functional rearrangement and expression of IgM heavy chain genes and is down-regulated when immature B cells mature and express IgD.5 It is expressed at a higher level on CD4+CD8+ thymocytes.5, 6 It is also expressed on cultured epidermal Langerhans cells.7 The biochemical features of murine CD25 have been characterized in detail.8-10. This antibody, used in combination with C2275-89A in culture, results in higher levels of inhibition of proliferation driven by IL-2 and generation of alloreactive CTL than either of these monoclonal antibodies alone. 2, 13 Applications: • Identification and enumeration of CD25+ cells by flow cytometry 1-4 • Immunoprecipitation 1, 2, 8, 10 • Inhibition of IL-2 or mitogen-induced T cell proliferation 13 • In vitro blocking of IL-2 binding 2, 12 Recommended Dilution: Flow Cytometry: ≤ 0.3ug/10e6 cells Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. APC is light sensitive.

Shipping Conditions:: Blue Ice