C2275-11D

CD24 (GPI-linked surface Mucin, Nectadrin) (PE)

Size

£307.00

SKU:: C2275-11D
Purity:: Purified
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Rat
Immunogen:: Mouse CD24/heat stable antigen (HSA)
Clone:: 30-F1
Format:: Supplied as a liquid in PBS, 0.09% sodium azide and a stabilizing agent. Labeled with R-Phycoerythrin (PE).
Extra Details:: CD24 is expressed in B cells until their terminal differentiation into plasma cells.1 It has been used widely as a marker to fractionate different B-lineage differentiation stages.10 CD24 has been found on a variety of other hematopoietic and neural cell types, and is now classified as a costimulatory molecule.2-4 The CD24/HSA molecule is composed mostly of carbohydrate, with a protein core of only 31-35 amino acids in the human and 27 amino acids in the mouse.1, 3 The molecular mass is variable, ranging from 38-70kD.3 CD24 is now included in the large group of phosphatidylinositol-anchored membrane proteins.1-4 Studies with purified CD24 applied to latex beads have demonstrated a self-binding property, suggesting that the molecule is capable of promoting homotypic aggregation, binding and cell adhesion.3 This is supported by earlier observations of the blockade of B cell lymphoblast aggregation with anti-CD24 monoclonal antibody.6 Recent studies of murine CD24 have assigned a role for CD24/HSA as a ligand for P-selectin, a calcium-dependent lectin.7 Applications: Suitable for use in Flow Cytometry, Immunoprecipitation and and cell sortin. Other applications not tested. Recommended Dilution: Flow Cytometry: ≤ 0.2ug/10e6 cells Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Shipping Conditions:: Blue Ice