C2273-91L
CD23 (IgE Receptor gp45) (PE)
Size
£704.00
- SKU:
- C2273-91L
- Purity:
- Purified by Protein A affinity chromatography from tissue culture supernatant.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Human peripheral myeloma cells
- Clone:
- BU38
- Format:
- Supplied as a liquid in PBS, pH 7.5, 0.2% BSA, 0.04% sodium azide, 15% glycerol. Labeled with R. Phycoerythrin (RPE)
- Extra Details:
- Human CD23 is a low affinity receptor for IgE (Fc epsilon RII) found primarily on mature B cells and also on eosinophils, monocytes, dendritic cells, platelets, and a subset of thymic epithelial cells. CD23 interacts with CD21. Applications: Suitable for use in Flow Cytometry, Immunohistochemistry and Western Blot. Other applications not tested. Recommended Dilutions: Flow Cytometry: 1:50 Immunohistochemistry: Formalin fixed paraffin embedded sections Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. Five x 10e5 ficoll prepared human peripheral white blood cells were washed and preincubated for 5 minutes with 20ul of 250ug/ml human IgG (to block non-specific binding) after which they were washed and incubated 45 min on ice with 80ul of C2273-91L at a 1:50 dilution. 2. Wash cells three times, fixed and analyzed using BD FACS calibur. 3. A net 13.6% sub-population of the cells were stained positive with a mean shift of 1.67 log10 flourescent units when compared to a IgG1 Murine (PE) negative control at a similar concentration. 4. Binding was 95% blocked when pre-incubatde 10 minutes with 20ul of 0.5mg/ml C2273-91H. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
- Shipping Conditions:
- Blue Ice