C2273-91C

CD23 (IgE Receptor gp45) (Biotin)

Size

£479.00

SKU:: C2273-91C
Purity:: Purified
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rat
Clone:: 2G8
Format:: Supplied as a liquid in PBS, 0.09% sodium azide. Labeled with Biotin.
Extra Details:: The CD23 antigen is the low affinity IgE Fc receptor, which is a 49kD protein with 38 and 28kD fragments.1 It is expressed on most mature, conventional B cells (but not on peritoneal CD5+ B cells), and can also be found on the surface of T cells, macrophages and platelets.1-6 It is distinct from the high affinity IgE receptors found on basophils and mast cells, which mediate allergic reactions. The low affinity receptors are thought to play a role in isotype specific immunoregulation.3 The regulation of CD23 surface expression appears to be integral with the complex IgE system, which involves interactions of cells, cytokines, antibodies and regulatory factors.2-6 CD23 has been described as a “membrane bound cytokine," in that the soluble cleavage products of CD23 are themselves able to act as cytokines in vitro.6 Applications: • Identification and enumeration of CD23+ cells by flow cytometry 2, 5 • Differentiation of B cell subpopulations by cell sorting 7 • Immunoprecipitation of CD23 1 Recommended Dilution: Flow Cytometry: ≤ 1ug/10e6 cells Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice