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C2273-02D-ML490

CD22 (CD22 Antigen, B Cell Receptor CD22 Precursor, B Lymphocyte Cell Adhesion Molecule, BL-CAM, Leu14, Lyb8, MGC130020, Sialic Acid Binding Immunoglobulin-like Lectin 2, Sialic Acid-binding Ig-like Lectin 2, Siglec 2, Siglec 2, Siglec 2) (MaxLight 490)

Size

£940.00

SKU:
C2273-02D-ML490
Purity:
Purified by Protein A affinity chromatography from tissue culture supernatant.
Storage Conditions:
4°C Do Not Freeze
Supplier:
United States Biological
Host:
Mouse
Immunogen:
Burkitt's lymphoma cell line Raji.
Clone:
MEM-01
Format:
Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™490.
Extra Details:
MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000. CD22 is initially expressed cytoplasmically and subsequently on the cell surface during B cell maturation. It is lost upon terminal differentiation to plasma cells. Applications: Suitable for use in Flow Cytometry, Immunoprecipitation, and Western Blot. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:100-1:200; 10ul labels 10e6 cells in 100ul Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
Shipping Conditions:
Blue Ice