CD22

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£544.00

SKU:: C2272-98F
Purity:: Purified by immunoaffinity chromatography. Endotoxin: ≤1EU/ug
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to human CD22 expressed in CHO cells.
Format:: Supplied as a lyophilized powder from PBS, pH 7.2. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.
Extra Details:: The CD22 antigen is only expressed on the cell surface of mature B-lymphocytes. The CD22 antigen is expressed on >90% of peripheral blood B-cells (1). CD22 is expressed on most peripheral B-cell lymphomas and leukemias including hairy cell leukemia, but not on the immature bone marrow type of leukemia. This antigen is not present on T-cell, monocytes or macrophages. Applications: Suitable for use in ELISA and Western Blot. Other applications have not been tested. Recommended Dilutions: ELISA (Sandwich): 0.5-2ug/ml using 100ul/well antibody solution) In conjunction with biotinylated anti-human CD22 (C2272-98G) as a detection antibody, allows the detection of at least 0.2-0.4ng/well of recombinant human CD22. Western Blot: 0.1-0.2ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant human CD22 is 1.5-3ng/lane, under either reducing or non-reducing conditions. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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