C2272-90D-FITC
CD22 (FITC)
Size
£960.00
- SKU:
- C2272-90D-FITC
- Application:
- Flow Cytometry
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rat
- Clone:
- 3H1116 (OX97)
- Format:
- Supplied as a liquid in PBS, pH 7.2. Labeled with Fluorescein isothiocyanate (FITC).
- Extra Details:
- CD22 is detected in the cytoplasm early in B cell development (late pro-B cell stage), appears on the cell surface simultaneously with surface IgD, and is found on most mature B cells. Expression is lost with terminal differentiation of B cells and is absent on plasma cells. Activation of B cells via surface Ig increases CD22 expression. CD22 reacts with most B cell leukemias including Hairy Cell Leukemia (HCL) and B cell lymphomas. Applications: Suitable for use in Flow Cytometry. Other applications have not been tested. Recommended Dilutions: Flow Cytometry: 1:50-1:100. Use 10ul to label 10e6 cells in 100ul. Optimal working dilutions to be determined by researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice