C2272-90D-BIOTIN

CD22 (Biotin)

Size

£960.00

SKU:: C2272-90D-BIOTIN
Application:: Flow Cytometry
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rat
Clone:: 3H1116 (OX97)
Format:: Supplied as a liquid in PBS, pH 7.2. Labeled with Biotin.
Extra Details:: CD22 is detected in the cytoplasm early in B cell development (late pro-B cell stage), appears on the cell surface simultaneously with surface IgD, and is found on most mature B cells. Expression is lost with terminal differentiation of B cells and is absent on plasma cells. Activation of B cells via surface Ig increases CD22 expression. CD22 reacts with most B cell leukemias including Hairy Cell Leukemia (HCL) and B cell lymphomas. Applications: Suitable for use in Flow Cytometry. Other applications have not been tested. Recommended Dilutions: Flow Cytometry: 1:50-1:100. Use 10ul to label 10e6 cells in 100ul. Optimal working dilutions to be determined by researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.